The Hotel San Juan de los Reyes is located in the Jewish Quarter in the historic district of central Toledo. It is near Santa Maria la Blanca Synagogue, the Museum of Victorio Macho, and the Saint Thomas Church. The Hotel San Juan de los Reyes can be accessed by car, and is about 90 minutes' drive from Madrid.

There are 38 rooms at the Hotel San Juan de los Reyes. The rooms have air conditioning, wireless Internet access, satellite television, direct dial telephones, mini bars, safe boxes, and en suite bathrooms. Some rooms offer views of the city, while others face the Cigarrales.

The Hotel San Juan de los Reyes is a converted flour-mill with a Neo-Mudejar façade dating back to the 19th century. It opened in 2003. A morning meal is offered in the breakfast room, and there is also a café offering small snacks and coffees throughout the day. A conference room can hold up to 200 people and has technology support systems for presentations. Free wireless Internet access is provided throughout the property. There is a gift shop on the main level, and there is a private parking garage for guests.